We aim to provide the most physiologically relevant human skin models using full thickness human skin explants prepared from surgical tissue, ethically sourced through the Norwich Biorepository. Our established models replicate complex cell-cell interactions between keratinocytes, fibroblasts, and resident immune cells as well as between these cells and the skin’s extensive extracellular matrix. These interactions can be disturbed in common skin conditions including atopic dermatitis (atopic eczema) and psoriasis as well as in healthy and impaired wound healing.